Introduce the Speakers of ASBA.
Sang Yup Lee, Ph.D. / Distinguished Professor / Department of Chemical and Biomolecular Engineering, KAIST
Our research field includes metabolic engineering, genomics/ proteomics/ metabolomics/bioinformatics, production of primary and secondary metabolites, production and engineering of proteins and other biopolymers, and DNA chip technology for diagnostics. A number of projects supported by government and companies have been completed, and many others are currently in progress. Our laboratory has made significant advances in the development of upstream-to-downstream processes for the production of various primary metabolites, recombinant proteins and biopolymers.Homepage
Ken-ichi Yoshida, Ph.D. / Professor / Kobe University
Inositol is cyclohexane-1,2,3,4,5,6-hexol, i.e. a hexane ring with a hydroxyl group on each carbon. Epimerization of the six hydroxyl groups makes nine inositol-stereoisomers. The most common of these stereoisomers in nature is myo-inositol, while another rare stereoisomer, scyllo-inositol, is known to reduce beta-amyloid accumulation in the brain to prevent Alzheimer's disease.
We aimed at production of scyllo-inositol from glucose in Bacillus subtilis. Glucose is incorporated as glucose 6-phosphate via the phosphotransferase system. myo-Inositol-1-phosphate synthase converts glucose 6-phosphate into myo-inositol-1-phosphate. This enzyme is not intrinsic to B. subtilis and thus must be introduced from the other organisms artificially, which was found to be active only when intracellular levels of NAD+/NADH were elevated by knocking-out purine efflux pump. The constitutive inositol monophosphatase dephosphorylates myo-inositol-1-phosphate into myo-inositol. Then myo-inositol was converted into scyllo-inositol by the engineered inositol metabolism. Thus, we here created a B. subtilis cell factory producing scyllo-inositol from glucose. Our next goal is to achieve further improvement in scyllo-inositol production.Homepage
Hirohide Saito, Ph.D. / Professor / Kyoto University
Gene delivery using RNA rather than DNA may be safer owing to a reduced risk of genomic integration. By designing microRNA (miRNA)-responsive synthetic mRNAs, (miRNA switches), weHomepage
developed a method for high-resolution identification, separation, and purification of target mammalian cells. The miRNA switches can control translation of transgenes depending on
endogenous miRNA activities and purify variety of target cells, including human pluripotent stem cells-derived cardiomyocytes and neurons with high efficiency and accuracy. Moreover, we prepared a set of “miRNA switch library” to rapidly identify active miRNA profiles in each cell type. Possible applications using new RNA technologies will be discussed.
Fan Jin, Ph.D. / Professor / University of Science and Technology of China
A significant opportunistic human pathogen, Pseudomonas aeruginosa establishes acute or chronic infections in patients, which turns out to be associated with the two switchable lifestyles of planktonic or free-living individuals and combined biofilm‐associated cells in responds to varying environments. Thriving adaptation to multiple environmental stimuli has been determined as a result of regulation of signaling systems in bacteria. To understand how pathgentic bacteria coordinate their gene expressions to adapt to host environments, we developed a series of high throughput techniques based on image processing, bacterial tracking and adaptive microscopy that allow us to profile and manipulate the phenotypes of single P. aeruginosa cells in a high throughput manner. Our results indicated that our methods can be used to identify or manipulate clinically relevant phenotypes in a mixed bacterial population, which is expected to be applied in microbiology and synthetic biology.Homepage
Akihiko Kondo, Ph.D. / Professor / Kobe University
Cost reduction of raw materials and processes is needed in order to use biomass as an alternative to fossil resources. Our team aims to integrating conventional processes, which are typically complicated and costly into a bio-process that is innovative, consistent, less costly and energy-saving. This will be achieved by optimizing, in an integrated manner, a plant's capacity to produce and degrade cellulose and the process of microorganisms' degrading and synthesizing biomass.Homepage